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    Conjugation of Basic Fibroblast Growth Factor on a Heparin Gradient for Regulating the Migration of Different Types of Cells

    Year: 2013

    Journal: Bioconjugate Chem., 2013, 24 (8), pp 1302–1313, 20131001

    Authors: Jindan Wu 1 2, Zhengwei Mao 1, Yifeng Hong 1, Lulu Han 1, and Changyou Gao * 1 3

    Last authors: Changyou Gao

    Organizations: 1 MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China 2 MOE Key Laboratory of Advanced Textile Materials & Manufacturing Technology, College of Materials and Textile, Zhejiang Sci-Tech University, Hangzhou 310018, China 3 State Key Laboratory of Diagnosis and Treatment for Infectious Diseases, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China

    Country: China

    Regulation of cell migration by cell growth factors is critical in tissue regeneration such as angiogenesis, wound healing, and bone formation. In this work, basic fibroblast growth factor (bFGF) with a density varying between 0 and 295 ng/cm2 was conjugated on heparinized glass slides. The amount of conjugated bFGF was determined by immunofluorescent staining. The mobility of vascular smooth muscle cells (VSMCs) was largely dominated by the bFGF density, whereas that of mesenchymal stem cells (MSCs) and endothelial cells (ECs) was slightly influenced. The migration rate of VSMCs increased initially and then decreased along with the increase of bFGF density. The fastest rate ( 22 μm/h) was found on the bFGF surface with a density of 83 ng/cm2. The intrinsic mechanisms of the diverse migration behaviors of the VSMCs, MSCs, and ECs were revealed by studying the expression of bFGF receptors and migration-related proteins. The results show that the cell mobility is regulated by complex and synergetic intracellular signals in a cell type-dependent manner.