Contact
    Start Publications Controlled Assembly of Protein in Glass Capillary
    ←  Publication Finder
    QSense

    Controlled Assembly of Protein in Glass Capillary

    Year: 2010

    Journal: Langmuir, 2010, 26 (15), pp 12803–12809, 20100831

    Authors: Lin Y. †‡, Su Z. †, Balizan E. ‡, Niu Z. *§, Wang Q. *‡

    Last authors: Qian Wang

    Organizations: † State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China ‡ Department of Chemistry and Biochemistry and Nanocenter, University of South Carolina, 631 Sumter Street, Columbia, South Carolina 29208 § Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100910, P. R. China

    Country: China

    By means of a slow drying process and the control of surface charge characteristics, protein stripe patterns were readily prepared on the luminal surface of a capillary. We systematically studied the effects of surface properties, pH, and protein concentration on pattern formation using optical microscopy, atomic force microscopy, and quartz crystal microbalance measurement. By balancing these parameters, a broad selection of proteins could be assembled within a capillary with well-defined stripe patterns. Neutravidin, one of the model proteins, was specifically chosen to demonstrate the bioactivity retained through the assembly process by interaction with fluorescently labeled biotin motifs. This technique therefore offers a facile approach for patterning proteins and other biomacromolecules in capillary tubes.