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    Crosslink Effect and Albumin Adsorption onto Chitosan/Alginate Multilayered Systems: An in situ QCM-D Study

    Year: 2010

    Journal: Macromolecular Bioscience, Volume 10, Issue 12, pages 1444–1455, December 8, 2010, 20110317

    Authors: Martins G.V. 1, 2 *, Merino E.G. 1 2, Mano J.F. 1 2, Alves N.M. 1 2

    Last authors: Natália M. Alves

    Organizations: 1 Department of Polymer Engineering, 3Bs Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Zona Industrial da Gandra, S. Cláudio do Barco 4806-909 Caldas das Taipas, Guimarães, Portugal 2 PT Government Associated Laboratory, IBB – Institute for Biotechnology and Bioengineering, Braga, Portugal

    Country: Portugal

    The adsorption of HSA onto CHI/ALG multilayer assemblies was assessed in situ using QCM-D. It was found that the behavior of HSA on biomaterials surface can be tuned by adjusting parameters of the polyelectrolyte system such as pH, layer number, crosslinker and polymer terminal layer. Our results confirmed the key role of electrostatic interactions during HSA adsorption, since oppositely charged surfaces were more effective in promoting protein adhesion. QCM-D data revealed that crosslinking (CHI/ALG)5CHI films allows HSA to become adsorbed in physiological conditions. Our results suggested that the biological potential of biopolymers and the mild conditions of the LbL technique turn these natural nanoassemblies into a suitable choice to be used as pH-sensitive coatings.