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    Development of a Biological Protocol for Endotoxin Detection Using Quartz Crystal Microbalance (QCM)

    Year: 2014

    Journal: Applied Biochemistry and Biotechnology, September 2014, 20141015

    Authors: E. Pérez-Lorenzo (1)(2), A. Zuzuarregui (1)(2)(3), S. Arana (1)(2), M. Mujika (1)(2)

    Last authors: M. Mujika

    Organizations: 1. CEIT-IK4 and Tecnun, University of Navarra, Paseo de Manuel Lardizábal 15, 20.018, Donostia-San Sebastián, Spain 2. CIC Microgune, Goiru kalea 9 Polo de Innovación Garaia, 20500, Arrasate-Mondragón, Spain 3. CIC NanoGUNE Consolider, Tolosa Hiribidea 76, 20018, Donostia-San Sebastian, Gipuzkoa, Spain

    Country: Spain

    In this paper, a biological protocol for endotoxin detection has been developed and optimized by quartz crystal microbalance (QCM). The parameters involved in the formation of the self-assembled monolayer (SAM) have been analyzed, and a study of the pH of the ligand buffer has been performed in order to find the best condition for the ligand immobilization and, in consequence, for the endotoxin detection. The detection limit obtained with the characterized biological protocol corresponds to 1.90 μg/ml. The effectiveness of the optimized biological protocol has been analyzed by cyclic voltammetry analysis.