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    Drying of DNA Droplets

    Year: 2006

    Journal: Langmuir 22 (2006), 6308-6312, 20111221

    Authors: Xiaohua Fang, Bingquan Li, Eric Petersen, Young-Soo Seo, Vladimir A. Samuilov, Yong Chen, Jonathan C. Sokolov, Chwen-Yang Shew, and Miriam H. Rafailovich

    Organizations: Department of Materials Science and Engineering, State University of New York at Stony Brook, New York 11794; Harvard University, Cambridge, Massachusetts 02138; Department of Chemistry, College of Staten Island, City University of New York, Staten Island, New York 10314

    The evaporation kinetics of droplets containing DNA was studied, as a function of DNA concentration. Drops containing very low DNA concentrations dried by maintaining a constant base, whereas those with high concentration dried with a constant contact angle. To understand this phenomenon, the distribution of the DNA inside the droplet was measured using confocal microscopy. The results indicated that the DNA was condensed mostly on the surface of the droplets. In the case of high concentration droplets, it formed a shell, whereas isolated islands were found for droplets of low DNA concentrations. Rheologic results indicate the formation of a hydro gel in the low concentration drops, whereas phase separation between the self-assembled DNA structures and the water phase occurred at higher concentration.