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    Dual Responsive Nanostructured Surfaces for Biomedical Applications

    Year: 2011

    Journal: Langmuir, 2011, 27 (13), pp 8415–8423, 20110906

    Authors: Martins G.V. †‡, Mano J.F †‡, Alves N.M *†‡

    Last authors: Natalia M. Alves

    Organizations: 3B's Research Group − Biomaterials, Biodegradables, and Biomimetics, Department of Polymer Engineering, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Zona Industrial da Gandra, S. Cludio do Barco, 4806-909 Caldas das Taipas Guimaraes, Portugal IBB − Institute for Biotechnology and Bioengineering, PT Government Associated Laboratory Braga, Portugal

    Country: Portugal

    In this paper, we describe the construction and characteristics of thermoresponsive, thin nanostructured films prepared by layer-by-layer sequential assembly of chitosan-graft-NIPAAm and alginate. FTIR and 1H NMR spectra have confirmed the introduction of NIPAAm moieties onto the chitosan backbone. The LCST of the synthesized copolymer was found to be around 31–33 °C. The formation of the polyelectrolyte multilayers containing the copolymer and alginate was followed in situ by quartz crystal microbalance with dissipation monitoring technique and ex situ by UV–vis measurements. Our results revealed the linear increase of the multilayer film growth and the influence of the presence of salt. Moreover, AFM analysis has confirmed that PNIPAAm is able to reconform upon temperature swaps even when combined with other layers in a polyelectrolyte multilayer, demonstrating that the nanoassemblies are thermoresponsive. Preliminary results showed that, upon reducing culture temperature below PNIPAAm LCST, a gradual detachment of cell sheets from these PNIPAAm-based coatings has occurred.