Contact
    Start Publications Effect of genipin cross-linking on the cellular adhesion ...
    ←  Publication Finder
    QSense

    Effect of genipin cross-linking on the cellular adhesion properties of layer-by-layer assembled polyelectrolyte films

    Year: 2009

    Journal: Biomaterials, Volume 30, Issue 27, September 2009, Pages 4463-4470, 20100827

    Authors: Hillberg A.L. 1 3, Holmes C.A. 1 3, Tabrizian M. 1 2 3

    Last authors: Maryam Tabriziana

    Organizations: aDepartment of Biomedical Engineering, McGill University, Duff Medical Building, 3775 University Street, Montreal, Quebec H3A 2B4, Canada bFaculty of Dentistry, McGill University, 3480 University Street, Montreal, Quebec H3A 2B2, Canada cCentre for Biorecognition and Biosensors (CBB), McGill University, Duff Medical Building, 3775 University Street, Montreal, Quebec H3A 2B4, Canada

    Country: Canada

    Use of polyelectrolyte multi-layers as biomaterials for cell attachment has been limited due to their gel-like characteristics. Herein, we attempt to improve the cellular adhesion properties of multi-layer films, reduce their gel-like nature and rigidify them through chemical cross-linking with genipin; a natural and non-cytotoxic compound. Chitosan (CH), hyaluronan (HA) and alginate (Alg) were used to assemble [CH–HA]n CH and [CH–Alg]n CH films, and the effects of genipin cross-linking on the cell adhesion properties of these multi-layers were investigated. Atomic force microscopy (AFM) confirmed that cross-linking affected each of the films differently. Quartz crystal microbalance with dissipation (QCM -D) revealed that [CH–HA]10 CH films were very viscoelastic, with thicknesses in the range 350–450 nm, while [CH–Alg]10 CH films only grew to thicknesses of 100 nm. These differences were a result of the different growth regimes of these two polyelectrolyte systems. Cell adhesion studies using MC3T3 pre-osteoblasts and rat fibroblastic skin cells, carried out on both films demonstrated vast differences in cell adhesion. [CH–HA]n CH cross-linked films proved to be highly non-adhesive for pre-osteoblasts and fibroblastic skin cells. Conversely, cross-linking [CH–Alg]n CH films was shown to dramatically improve pre-osteoblast and rat fibroblastic skin cell adhesion, especially for high bi-layer numbers and using higher concentrations of cross-linker.