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    Effect of Surface Parameters on the Performance of IgG-Arrayed Hydrogel Chips: A Comprehensive Study

    Year: 2007

    Journal: Langmuir, 2007, 23 (21), pp 10551–10558, 20111221

    Authors: Katarzyna Derwinska, Levi A. Gheber, Ursula Sauer, Leopold Schorn, and Claudia Preininger

    Organizations: Austrian Research Centers GmbH - ARC, Departments of Bioresources and Materials Research, 2444 Seibersdorf, Austria, and Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel

    In this article, the assay performance of 3D polyurethane (PU) hydrogel surfaces, used either plain or modified with cross linkers and additives in a direct immunoassay for IgG, is correlated with chip surface parameters such as water content and expansion, mechanical stability, hydrophilicity, thickness, and surface topography. Commercial chip surfaces ARChip Epoxy, Nexterion slide H and HydroGel are used as references. A strong correlation between assay sensitivity and physical surface parameters was found only for various hydrogels of the same chemical composition, in which cases assay sensitivity increases with decreasing hydrogel concentration as well as decreasing roughness, water content, and expansion. However, as is the case with all hydrogels tested, more hydrophobic layers with low water content are more highly reproducible from one measurement to another.