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    Efficient transfer of human adipose-derived stem cells by chitosan/gelatin blend films

    Year: 2012

    Journal: Journal of Biomedical materials research, Part B Applied biomaterials 2012, 100B (5) pp 1369-1377, 20121211

    Authors: Nai-Chen Cheng, Hsu-Hsien Chang, Yuan-Kun Tu, Tai-Horng Young

    Organizations: Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan, Department of Surgery, National Taiwan University Hospital and College of Medicine, Taipei 100, Taiwan, Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, Taipei 100, Taiwan, Department of Orthopedics, E-Da Hospital/I-Shou University, Kaohsiung, Taiwan

    Adipose-derived stem cells (ASCs) are a potential source of abundant mesenchymal stem cells and represent a promising cell-based therapy for tissue damage or degeneration conditions. Previous investigations have demonstrated enhanced therapeutic effects of ASCs in a three-dimensional spheroid culture formulation. In this study, we hypothesize that a composite membrane made of chitosan/gelatin (C/G) is beneficial to facilitate transfer of human ASCs in spheroids. Increasing chitosan content within the blends enhanced the mechanical properties of the sample, including tensile strength and elongation-at-break ratio. Although ASC spheroids developed shortly after seeding on pure chitosan films, increasing gelatin proportion in the C/G blends promoted cell adhesion onto the membranes. We also found that ASCs did not proliferate on chitosan films, but C/G blends of different ratios supported ASC proliferation in the first 4 days of culture. However, ASCs on all C/G blends started to detach from the films to form spheroids after day 4, while ASCs on pure gelatin films remained attached and continued to grow. Gradual gelatin release from the C/G blend films, leading to enriched chitosan content in the blends, probably encouraged ASC detachment and spheroid formation. We placed porous collagen matrix on ASC-seeded C/G blends to simulate the application of ASC-seeded C/G films onto injured tissue and found that a C/G film composed of 75% chitosan could facilitate significantly more cell transfer into the overlying collagen sponge. Therefore, a blend film containing 75% chitosan and 25% gelatin showed promising results to serve as a biomaterial for human ASC-based cell therapy.