Contact
    Start Publications Electrochemical detection of DNA hybridization using ...
    ←  Publication Finder
    QSense

    Electrochemical detection of DNA hybridization using metallacarborane unit

    Year: 2012

    Journal: Bioelectrochemistry Volume 87, October 2012, Pages 78–83, 20130117

    Authors: Robert Ziółkowski a, Agnieszka B. Olejniczak b, Łukasz Górski a, Justyna Janusik a, Zbigniew J. Leśnikowski b, Elżbieta Malinowska a

    Last authors: Elżbieta Malinowska

    Organizations: a Warsaw University of Technology, Faculty of Chemistry, Institute of Biotechnology, Department of Microbioanalytics, Noakowskiego 3, 00–664 Warsaw, Poland b Institute for Medical Biology, Laboratory of Molecular Virology and Biological Chemistry, Polish Academy of Sciences, Lodowa 106, 93-232 Lodz, Poland

    Country: poland

    The evaluation of novel electrochemically active label for electrochemical detection of DNA hybridization is presented. Metallacarborane units modified with iron, cobalt or chromium were investigated. The value of redox potential and relatively strong current signal facilitate usage of Fe-carborane as marker covalently attached to the ssDNA. In electrochemical genosensor the sequence complementary to UL55 gene was labeled and used as a target for biosensor device. Interactions were investigated using electrochemical and piezoelectric methods. Obtained results confirm usefulness of the designed label in electrochemical detection of DNA hybridization.