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    Energy dissipation kinetics for protein and antibody-antigen adsorption under shear oscillation on a quartz crystal microbalance

    Year: 1998

    Journal: Langmuir 1998 vol 14, No 4, p 729-734, 20100827

    Authors: Höök F. 1 2, Rodahl M. 1, Brzezinski P. 2, Kasemo B. 1

    Last authors: Bengt Kasemo

    Organizations: 1 Department of Applied Physics, Chalmers University of Technology and Göteborg University, S-412 96 Göteborg, Sweden 2 Department of Biochemistry and Biophysics; Lundberg Laboratory, Medicinareg 9C, Chalmers University of Technology and Göteborg University, S-41390 Göteborg, Sweden

    Country: Sweden

    A new quartz crystal microbalance (QCM) instrument, allowing simultaneous frequency (f) and dissipation factor (D) measurements, has been used to study protein adsorption kinetics by measuring time-resolved data of both the D-factor, measuring the energy dissipation due to the added overlayer, and the f-shift, measuring the effective mass load on the sensor. Four model proteins (myoglobin (Mb), hemoglobin (Hb), human serum albumin (HSA), ferritin (Fer)) and one antibody-antigen reaction (antibody against HSA) were studied on a hydrophobic, methyl terminated (-CH3) gold surface. In all five cases system-specific, positive D-shifts and negative f-shifts were observed, revealing different adsorption phases. The D-factor measurements provide new information about protein adsorption and improves the interpretation of the frequency shift in terms of mass uptake. Possible mechanisms for the adlayer-induced dissipation are discussed.