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    Enzymatic Cross-Linking of a Phenolic Polymer Extracted from the Marine Alga Fucus serratus

    Year: 2004

    Journal: Biomacromolecules 2004, 5, 2376-2383, 20100827

    Authors: Berglin M., Delage L., Potin P., Vilter H., Elwing H.

    Last authors: Hans Elwing

    Organizations: Cell and Molecular Biology, Interface Biophysics, Göteborg University, Box 462, SE-405 30 Sweden, UMR 7139 CNRS-GOEMAR-UPMC, Station Biologique, BP 74, F-29682 Roscoff Cedex, France, and Zurmaierstrasse 19, D-54292 Trier, Germany

    Country: Sweden

    We have shown that a phenolic polymer (PP) extracted from Fucus serratus can be cross-linked using a vanadium-dependent bromoperoxidase (BPO). The methanol extracted PP was adsorbed to a quartz crystal sensor and the cross-linking was initiated by the addition of BPO, KBr, and H2O2. The decreased dissipation upon addition of the cross-linking agents, as measured with the quartz crystal microbalance with dissipation monitoring (QCM-D) method, was interpreted as intramolecular cross-links were formed between different phloroglucinol units in the PP. With surface plasmon resonance, it was shown that no desorption occurred from the sensor surface during the cross-linking. UV/vis spectroscopy verified the results achieved with QCM-D that all components, i.e., BPO, KBr, and H2O2, were necessary in order to achieve intramolecular oxidative cross-linking of the polymer.