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    Fibrinogen adsorption and platelet adhesion to silica surfaces with stochastic nanotopography

    Year: 2014

    Journal: BIOINTERPHASES, 9, 041002 (2014), 20150430

    Authors: Lord, Megan S.; Whitelock, John M.; Simmons, Anne; Williams, Rachel L.; Milthorpe, Bruce K.

    Organizations: Univ New S Wales, Grad Sch Biomed Engn, Sydney, NSW 2052, Australia; Univ New S Wales, Sch Mech & Mfg Engn, Sydney, NSW 2052, Australia; Univ Liverpool, Inst Ageing & Chron Dis, Dept Eye & Vis Sci, Liverpool L69 3GA, Merseyside, England; Univ Technol Sydney, Fac Sci, Broadway, NSW 2007, Australia

    In this study, the effect of surface nanoscale roughness on fibrinogen adsorption and platelet adhesion was investigated. Nanorough silica surfaces with a low level of surface roughness (10 nm R-rms) were found to support the same level of fibrinogen adsorption as the planar silica surfaces, while nanorough silica surfaces with higher levels of surface roughness (15 nm R-rms) were found to support significantly less fibrinogen adsorption. All surfaces analyzed were found to support the same level of platelet adhesion; however, platelets were rounded in morphology on the nanorough silica surfaces while platelets were spread with a well-developed actin cytoskeleton on the planar silica. Unique quartz crystal microbalance with dissipation monitoring (QCM-D) responses was observed for the interactions between platelets and each of the surfaces. The QCM-D data indicated that platelets were more weakly attached to the nanorough silica surfaces compared with the planar silica. These data support the role of surface nanotopography in directing platelet-surface interactions even when the adsorbed fibrinogen layer is able to support the same level of platelet adhesion. (C) 2014 American Vacuum Society.