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    Fibronectin adsorption on gold, Ti-, and Ta-oxide investigated by QCM-D and RSA modelling

    Year: 2008

    Journal: Journal of Colloid and Interface Science 320 (2008) 110–116, 20100827

    Authors: Hemmersam A.G., Rechendorff K., Foss M., Sutherland D.S, Besenbacher F.

    Last authors: Flemming Besenbacher

    Organizations: Interdisciplinary Nanoscience Center (iNANO) and Department of Physics and Astronomy, University of Aarhus, 8000 Aarhus C, Denmark

    Country: Denmark

    The adsorption of fibronectin on gold, Ti-, and Ta-oxide surfaces is investigated by means of the quartz crystal microbalance with dissipation (QCM-D) technique. The surface chemistry (gold, Ti-, and Ta-oxide) is found to influence the frequency shift observed during adsorption of the fibronectin layer with the magnitude being delta f Au >delta f Ti-oxide ~ delta f Ta-oxide. Corresponding variations in the dissipation change normalised to frequency change (delta D/delta f ) for the layer are observed. The QCM-D data are further analyzed by the random sequential adsorption (RSA) model, and adsorption rate parameter ka and footprint (a) determined, which supported the trend seen in the delta f and delta D/delta f values. The value of ka found by the RSA modelling of the QCM-D resonance frequency data is found to match the ratio between the mass measured by QCM-D and the mass reported by optical techniques in literature. We conclude that comparison of the adsorption rate parameter (ka) obtained by RSA modelling of the QCM-D data with ka values obtained from RSA modelling of data obtained using optical techniques can be a route to determine the degree
    of hydration of the adsorbed protein layer.