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    Fluid Biomembranes Supported on Nanoporous Aerogel/Xerogel Substrates

    Year: 2004

    Journal: Langmuir 2004, 20, 7232-7239, 20100827

    Authors: Weng K.C., Stålgren J.J.R., Duval D.J., Risbud S.H., Frank C.W.

    Last authors: Curtis W. Frank

    Organizations: Department of Materials Science and Engineering, Stanford University, Stanford, California 94305, Department of Chemical Engineering, Stanford University, Stanford, California 94305, Department of Chemical Engineering and Materials Science, University of California at Davis, Davis, California 95616, and Center on Polymer Interfaces and Macromolecular Assemblies (CPIMA), Stanford, California 94305

    Country: USA, US, United States of America

    Planar supported lipid bilayers have attracted immense interest for their properties as model cell membranes and for potential applications in biosensors and lab-on-a-chip devices.Wereport the formation of fluid planar biomembranes on hydrophilic silica aerogels and xerogels. Scanning electron microscopy results showed the presence of interconnected silica beads of approximately 10-25 nm in diameter and nanoscale open pores of comparable size for the aerogel and grain size of ~36-104 nm with ~9-24 nm diameter pores for the xerogel. When the aerogel/xerogel was prehydrated and then allowed to incubate in L-a-phosphatidylcholine (egg yolk PC) unilamellar vesicle (~30 nm diameter) solution, lipid bilayers were formed due to the favorable interaction of vesicles with the hydroxyl-abundant silica surface. Lateral mobility of labeled lipid N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3 phosphoethanolamine was retained in the membranes. A diffusion coefficient of 0.61 ±0.22 mm2/s was determined from fluorescence recovery after photobleaching analysis for membranes on aerogels, compared to 2.46 ± 0.35 mm2/s on flat glass. Quartz crystal microbalance-dissipation was utilized to monitor the kinetics of the irreversible adsorption and fusion of vesicles into bilayers on xerogel thin films.