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    Formation of Nanopore-Spanning Lipid Bilayers through Liposome Fusion

    Year: 2011

    Journal: Langmuir, 2011, 27 (17), pp 10920–10928, 20110906

    Authors: Kumar K. †, Isa L.†, Egner A. ‡, Schmidt R. ‡, Textor M. †, Reimhult E. *

    Last authors: Erik Reimhult

    Organizations: Department of Materials, Laboratory for Surface Science and Technology, Swiss Federal Institute of Technology (ETH Zrich), CH-8093 Zrich, Switzerland Department of NanoBioPhotonics, Max-Planck Institute, D-37077 Gttingen, Germany Department of Nanobiotechnology, University of Natural Resources and Life Sciences (BOKU) Vienna, A-1190 Vienna, Austria

    Country: Switzerland, Germany, Austria

    Self-assembly of nanopore-spanning lipid bilayers (npsLBs) paves the way toward chip-based integrated membrane protein biosensing. We present a novel approach to analyze the formation of npsLB at individual nanopores using quantitative analysis of high-resolution microscopy images. From this analysis we derive necessary conditions for the formation of npsLBs on nanopore arrays by liposome fusion and discuss the limitations of the process as a function of nanopore geometry, lipid membrane properties, and surface interaction. Most importantly, applying liposomes with diameters larger than the nanopore is demonstrated to be a necessary but not sufficient condition for npsLB formation. A theoretical model is used to discuss and explain this experimental finding.