Contact
    Start Publications Fusion of Bolaamphiphile Micelles: A Method to Prepare Stable ...
    ←  Publication Finder
    QSense

    Fusion of Bolaamphiphile Micelles: A Method to Prepare Stable Supported Biomimetic Membranes

    Year: 2013

    Journal: Langmuir, 2013, 29 (4), pp 1152–1161, 20130403

    Authors: Y. Kaufman 1 2, S. Grinberg 3, C. Linder 4 2, E. Heldman 5, J. Gilron 2, and V. Freger *6

    Last authors: V. Freger

    Organizations: 1 Albert Katz International School for Desert Studies and Unit of Environmental Engineering, Ben-Gurion University of the Negev, Sde-Boqer, Israel 3 Department of Chemistry, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel 4 Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel 5 Department of Clinical Biochemistry, the Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel 2 Zuckerberg Institute for Water Research, Ben-Gurion University of the Negev, Sde-Boqer, 84990, Israel 6 Wolfson Department of Chemical Engineering, Technion − Israel Institutes of Technology, Haifa 32000, Israel

    Country: Israel

    Supported biomimetic membranes (SBMs) on solid substrates have been commonly prepared from vesicle-forming double-tail lipids, such as zwitterionic phospholipids, using the method of vesicle fusion. Here we report on the preparation of SBMs on silica surfaces via a similar process of “micelle fusion” from a cationic single-tail bolaamphiphile GLH-20 that forms spherical and elongated thread-like micelles in solution. We demonstrate that, in contrast to zwitterionic phospholipids, GLH-20 self-assembles into a stable contiguous SBM at both low and high ionic strengths. The cationic charge of GLH-20 promotes the formation of a stable SBM through enhanced double-layer interactions with the negatively charged silica surface. It is also shown that spinach aquaporin PM-28 was successfully incorporated within bolaamphiphile SBM in a manner similar to SBMs prepared by vesicle/proteoliposome fusion; thereby the inherent curvature of the micelle surface does not inhibit protein reconstitution. The results suggest that SBMs based on charged bolaamphiphiles might be an attractive platform for applications such as water purification and biosensors, where the stability and low defect rate of SBMs in diverse conditions are crucial for achieving desired performance.