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    Gold nanoparticle-enhanced secondary ion mass spectrometry imaging of peptides on self-assembled monolayers

    Year: 2006

    Journal: Anal. Chem. 2006, 78, 1913-1920, 20100827

    Authors: Kim Y-P., Oh E., Hong M-Y., Lee D., Han M-K-, Shon H.K., Moon D.W., Kim H-S., Lee T.G.

    Last authors: Tae Geol Lee

    Organizations: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Kusung-Dong, Yusung-Gu, Daejeon 305-701, Korea, and Nano-Surface Group, Korea Research Institute of Standards and Science, Daejeon 305-600, Korea

    Country: Korea

    We demonstrate the use of gold nanoparticles (AuNPs) to enhance the secondary ion emission of peptides in time-of-flight secondary ion mass spectrometry (TOF-SIMS). The signal intensity of peptides adsorbed onto AuNPs was significantly increased when compared to that of self-assembled monolayers (SAMs). This gold nanoparticle-enhanced SIMS, termed NE-SIMS, enabled the sensitive detection of subtle modifications of peptides, such as phosphorylation. From a quantitative analysis of the amounts of adsorbed peptides and AuNPs on SAMs using quartz crystal microbalance and surface plasmon resonance spectroscopy, the ratio of peptide molecule to AuNP on amine-SAMs was revealed to be 18-19:1. When considering the ratio of peptide to matrix (1:10(3)-10(6)) employed in a matrix-enhanced SIMS, the use of AuNPs gave rise to a significantly increased secondary ion emission of peptides. Peptides were adsorbed onto patterned AuNPs on SAMs using a microfluidic system, and well-contrasted molecular ion images were obtained. NE-SIMS is expected to be applied to a chip-based analysis of modification of biomolecules in a label-free manner.