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    High-resolution imaging of a cellattached nanointerface using a gold-nanoparticle two-dimensional sheet

    Year: 2017

    Journal: Sci Rep, Volume 7, JUN 16

    Authors: Masuda, Shihomi; Yanase, Yuhki; Usukura, Eiji; Ryuzaki, Sou; Wang, Pangpang; Okamoto, Koichi; Kuboki, Thasaneeya; Kidoaki, Satoru; Tamada, Kaoru

    Organizations: NEXT Program; JSPS KAKENHI in Japan [26246005]

    This paper proposes a simple, effective, non-scanning method for the visualization of a cell-attached nanointerface. The method uses localized surface plasmon resonance (LSPR) excited homogeneously on a two-dimensional (2D) self-assembled gold-nanoparticle sheet. The LSPR of the gold-nanoparticle sheet provides high-contrast interfacial images due to the confined light within a region a few tens of nanometers from the particles and the enhancement of fluorescence. Test experiments on rat basophilic leukemia (RBL-2H3) cells with fluorescence-labeled actin filaments revealed high axial and lateral resolution even under a regular epifluorescence microscope, which produced higher quality images than those captured under a total internal reflection fluorescence (TIRF) microscope. This non-scanning-type, high-resolution imaging method will be an effective tool for monitoring interfacial phenomena that exhibit relatively rapid reaction kinetics in various cellular and molecular dynamics.
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