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    Hyaluronic acid / chitosan multilayer coatings on neuronal implants for localized delivery of siRNA nanoplexes

    Year: 2013

    Journal: Journal of Controlled Release Volume 168, Issue 3, 28 June 2013, Pages 289–297, 20130629

    Authors: Hanna Hartmann a 1, Susanne Hossfeld a 1, Burkhard Schlosshauer a, Ursula Mittnacht a, Ana Paula Pêgo c, Martin Dauner d, Michael Doser d, Dieter Stoll a b, Rumen Krastev a

    Last authors: Rumen Krastev

    Organizations: a NMI Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, Germany b Albstadt-Sigmaringen University, Anton-Günther-Str. 51, 72488 Sigmaringen, Germany c INEB Instituto de Engenharia Biomédica, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal d ITV Institute of Textile Technology and Process Engineering, Körschtalstr. 26, 73770 Denkendorf, Germany

    Country: Germany, Portugal

    Binding, stabilizing and promoting cellular uptake of siRNA are all critical efforts in creating matrices for the localized delivery of siRNA molecules to target cells. In this study, we describe the generation of chitosan imidazole/siRNA nanoplexes (NPs) embedded in nano scope polyelectrolyte multilayers (PEMs) composed of hyaluronic acid and chitosan for sustained and localized drug delivery. Regular PEM build-up, successful integration of NPs and controlled release under physiological conditions were shown. Biological efficacy was evaluated in neuronal cell culture concerning cell adhesion, viability, NPs uptake and gene silencing. The additionally shown biological functionalization of neuronal implants possesses potential for future applications in the field of regenerative medicine and treatment of spinal cord injuries.