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    Hydrogenase-poly(viologen) complex monolayers and electrochemical properties in Langmuir-Blodgett films

    Year: 2002

    Journal: Colloids and Surfaces
    A: Physicochemical and Engineering Aspects 198-200 (2002) 663-669, 20111221

    Authors: D.J. Qian , C. Nakamura , N. Zorin , J. Miyake

    Organizations: a National Institute of Advanced Interdisciplinary Research, AIST, 1- 1- 4 Higashi, Tsukuba, Ibaraki 305, Japan b Laboratory of Biochemistry and Biotechnology of Photosynthetic Microorganisms, Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia

    Mixed monolayers of a hydrogenase (H2ase) purified from Thiocapsa roseopersicina and poly(viologen) or hexadecyltrimethylammonium bromide (HTAB) were formed at the air-subphase interface. A larger H2ase-occupied area was measured in the H2ase-poly(viologen) or in the H2ase-HTAB mixed monolayers than that in the pure H2ase monolayer. These monolayers were transferred onto solid supports by the Langmuir-Blodgett (LB) method. Spectroscopic measurement indicated that H2ase activity was retained in the LB films. Cyclic voltammograms (CVs) of the H2ase-HTAB LB film showed two waves at ~-560 and ~-530 mV corresponding to the reduction and oxidation potentials of the [4Fe-4S]2+/1+ clusters of H2ase. For the H2ase-poly(viologen) LB films, the CV curves showed two broad waves centered at ~-510 and ~-480 mV, which correspond to the reversible one-electron process of poly(viologen)2+/1+ with a small contribution of the [4Fe-4S]2+/1+ clusters of H2ase. Charge transfer properties of the modified electrodes are discussed by use of chronocoulometry.