IgG1-glycolipidic LB films obtained by vertical deposition of an interfacial film formed through proteo-liposome spreading at the air/water interface
This study focuses on the formation of an immunoglobulin-glycolipid interfacial film through the disintegration of proteo-glycolipid liposomes at the air/buffer interface. The IgG-glycolipid liposomes were formed by mechanical dispersion and were spread on the buffer surface. The time-dependent increase in surface pressure following the liposome spreading was attributed to the formation of a surface film due to the interfacial reorganisation of the proteo-glycolipid vesicles. The presence of immunoglobulin in the glycolipid matrix was evidenced by the modification of the elastic properties of the interfacial film during the film compression. The transfer efficiency of the immunoglobulin with the glycolipid matrix by vertical Langmuir-Blodgett (LB) deposition procedure has been characterised through ATR-FTIR and fluorescence spectroscopies. Observations performed with Epi-fluorescence microscopy gives a direct evidence of the presence of immunoglobulin in the LB film.