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    Imaging interactions of cationic antimicrobial peptides with model lipid monolayers using X-ray spectromicroscopy

    Year: 2011

    Journal: Eur Biophys J, 2011, 40 (6), 805-810, 20131009

    Authors: Bonnie O. Leung, Adam P. Hitchcock, Amy Won, Anatoli Ianoul, Andreas Scholl

    Organizations: Department of Chemistry and Chemical Biology, McMaster University, Hamilton, ON, L8S 4M1, Canada; Department of Chemistry, Carleton University, Ottawa, ON, K1S 5B6, Canada; Advanced Light Source, Berkeley Lab, Berkeley, CA, 94720, USA

    The interaction of antimicrobial peptide anoplin with 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] lipid monolayers was imaged with atomic force microscopy, scanning transmission X-ray microscopy, and X-ray photoemission electron microscopy. X-ray absorption spectromicroscopy of the surface revealed the domains of the phase-segregated surface to be composed of 98(±5)% lipid while the matrix consisted of a ~50:50 lipid-peptide mixture. We show X-ray spectromicroscopy to be a valuable quantitative tool for label-free imaging of lipid monolayers with antimicrobial peptides at a lateral spatial resolution below 80 nm.