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    Imaging Macromolecular Interactions at an Interface

    Year: 2010

    Journal: Langmuir, 2010, 26 (4), 2452–2459, 20131009

    Authors: Joshua W. Lampe, Zhengzheng Liao, Ivan J. Dmochowski, Portonovo S. Ayyaswamy, David M. Eckmann

    Organizations: Center for Resuscitation Science, Department of Emergency Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA; Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA; Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA; Department of Anesthesiology and Critical Care, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA

    Important physiological, pathological, and technological processes occur at continuous and dispersed phase interfaces. Understanding these processes is limited by inability to quantitate molecular events occurring at the interface. To provide a model-independent measurement of protein concentration and mobility at the interface, we employed confocal laser scanning microscopy (CLSM). Fluorescently labeled albumin and fibrinogen were studied singly, pairwise, and with a surfactant, Pluronic F-127, in aqueous droplets. CLSM enables measurement of molecular behaviors manifest as surface inhomogeneity and of biophysical quantities including partitioning between the bulk and the gas−liquid (GL) interface. We conclude that albumin and fibrinogen behave substantially differently at the GL interface, adsorption from multispecies solutions is fundamentally different than adsorption from solutions of single species, and surfactants can inhibit proteins from occupying the interface.