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    Inhibitory effect of lignin during cellulose bioconversion: The effect of lignin chemistry on non-productive enzyme adsorption

    Year: 2013

    Journal: Bioresource Technology Volume 133, April 2013, Pages 270–278, 20130402

    Authors: Jenni L. Rahikainen 1, Raquel Martin-Sampedro 2, Harri Heikkinen 1, Stella Rovio 1, Kaisa Marjamaa 1, Tarja Tamminen 1, Orlando J. Rojas 2 3 4, Kristiina Kruus 1

    Last authors: Kristiina Kruus

    Organizations: 1 VTT Technical Research Centre of Finland, P.O. Box 1000, 02044 VTT, Finland 2 Aalto University, Department of Forest Products Technology, P.O. Box 16300, 00076 Aalto, Finland 3 Department of Forest Biomaterials, North Carolina State University, Raleigh, NC 27695, USA 4 Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA

    Country: Finland, USA, US, United States, United States of America, America

    The effect of lignin as an inhibitory biopolymer for the enzymatic hydrolysis of lignocellulosic biomass was studied; specially addressing the role of lignin in non-productive enzyme adsorption. Botanical origin and biomass pre-treatment give rise to differences in lignin structure and the effect of these differences on enzyme binding and inhibition were elucidated. Lignin was isolated from steam explosion (SE) pre-treated and non-treated spruce and wheat straw and used for the preparation of ultrathin films for enzyme binding studies. Binding of Trichoderma reesei Cel7A (CBHI) and the corresponding Cel7A-core, lacking the linker and the cellulose-binding domain, to the lignin films was monitored using a quartz crystal microbalance (QCM). SE pre-treatment altered the lignin structure, leading to increased enzyme adsorption. Thus, the positive effect of SE pre-treatment, opening the cell wall matrix to make polysaccharides more accessible, may be compromised by the structural changes of lignin that increase non-productive enzyme adsorption.