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    Inhibitory effect of lignin during cellulose bioconversion: The effect of lignin chemistry on non-productive enzyme adsorption

    Year: 2013

    Journal: Bioresource Technology, 2013, 133, pp 270-278, 20130926

    Authors: Jenni L. Rahikainen, Raquel Martin-Sampedro, Harri Heikkinen, Stella Rovio, Kaisa Marjamaa, Tarja Tamminen, Orlando J. Rojas, Kristiina Kruus

    Organizations: VTT Technical Research Centre of Finland, P.O. Box 1000, 02044 VTT, Finland; Aalto University, Department of Forest Products Technology, P.O. Box 16300, 00076 Aalto, Finland; Department of Forest Biomaterials, North Carolina State University, Raleigh, NC 27695, USA; Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA

    The effect of lignin as an inhibitory biopolymer for the enzymatic hydrolysis of lignocellulosic biomass was studied; specially addressing the role of lignin in non-productive enzyme adsorption. Botanical origin and biomass pre-treatment give rise to differences in lignin structure and the effect of these differences on enzyme binding and inhibition were elucidated. Lignin was isolated from steam explosion (SE) pre-treated and non-treated spruce and wheat straw and used for the preparation of ultrathin films for enzyme binding studies. Binding of Trichoderma reesei Cel7A (CBHI) and the corresponding Cel7A-core, lacking the linker and the cellulose-binding domain, to the lignin films was monitored using a quartz crystal microbalance (QCM). SE pre-treatment altered the lignin structure, leading to increased enzyme adsorption. Thus, the positive effect of SE pre-treatment, opening the cell wall matrix to make polysaccharides more accessible, may be compromised by the structural changes of lignin that increase non-productive enzyme adsorption.