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    Interaction of Human Plasma Proteins with Thin Gelatin-Based Hydrogel Films: A QCM-D and ToF-SIMS Study

    Year: 2014

    Journal: Biomacromolecules, 2014, 15 (7), pp 2398–2406, 20141008

    Authors: Sina M. S. Schönwälder †, Florence Bally †‡, Lars Heinke †, Carlos Azucena †, Özgül D. Bulut †, Stefan Heißler †, Frank Kirschhöfer †, Tim P. Gebauer §∥,Axel T. Neffe §∥, Andreas Lendlein §∥, Gerald Brenner-Weiß †, Jörg Lahann †, Alexander Welle †⊥,Jörg Overhage †, and Christof Wöll †

    Last authors: Christof Wöll

    Organizations: † Karlsruhe Institute of Technology (KIT), Institute of Functional Interfaces (IFG), 76344 Eggenstein-Leopoldshafen, Germany ‡ University of Upper Alsace (UHA), Institute of Materials Science of Mulhouse (IS2M, UMR 7361), 68093 Mulhouse, France § Helmholtz-Zentrum Geesthacht, Institute of Biomaterial Science, 14513 Teltow, Germany ∥ Helmholtz Virtual Institute - Multifunctional Biomaterials for Medicine, Teltow, Berlin, Germany ⊥ Karlsruhe Nano Micro Facility (KNMF), Karlsruhe Institute of Technology (KIT), 76344 Eggenstein-Leopoldshafen, Germany

    Country: Germany, France

    In the fields of surgery and regenerative medicine, it is crucial to understand the interactions of proteins with the biomaterials used as implants. Protein adsorption directly influences cell-material interactions in vivo and, as a result, regulates, for example, cell adhesion on the surface of the implant. Therefore, the development of suitable analytical techniques together with well-defined model systems allowing for the detection, characterization, and quantification of protein adsorbates is essential. In this study, a protocol for the deposition of highly stable, thin gelatin-based films on various substrates has been developed. The hydrogel films were characterized morphologically and chemically. Due to the obtained low thickness of the hydrogel layer, this setup allowed for a quantitative study on the interaction of human proteins (albumin and fibrinogen) with the hydrogel by Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D). This technique enables the determination of adsorbant mass and changes in the shear modulus of the hydrogel layer upon adsorption of human proteins. Furthermore, Secondary Ion Mass Spectrometry and principal component analysis was applied to monitor the changed composition of the topmost adsorbate layer. This approach opens interesting perspectives for a sensitive screening of viscoelastic biomaterials that could be used for regenerative medicine.