Langmuir–Blodgett film based biosensor for estimation of phenol derivatives

The structure and biosensor characteristic of complex between phenoloxidases (laccase, tyrosinase) and lipid-like thin ordered layer were investigated. The advantageous of immobilization method based on Langmuir–Blodgett film as a matrix has been developed for characterization of sensing protein layers effect. Laccase from Cerrena unicolor and tyrosinase from Agaricus bisporus molecules were densely adsorbed onto Langmuir monolayer surface and then deposited on solid support which can be used for phenolic compounds determination. The function of enzyme immobilization was carried by adsorption process. Phenoloxidases were adsorbed on monolayer built of linoleic acid and, octadecyltrimethylammonium bromide. The sensor sensitization was achieved by an amphiphilic conducting polymer—poly[(N-nonylphenoxazine-3,7-diyl-alt-(1,2,3-benzothiadiazole)] admixed into the film. The interlaced polymer was expected to facilitate the electron transfer as well enhancing the sensor sensitivity. Considering the fact, that immobilization strategy showed high efficiency, obtained results suggest that our method for phenoloxidases immobilization has a great potential of enabling high throughput fabrication of bioelectronics’ devices.