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    Large Area Protein Nanopatterning for Biological Applications

    Year: 2006

    Journal: Nano Lett., Vol. 6, No. 6, 2006 1165-1171, 20100827

    Authors: Agheli H., Malmström J., Larsson E. M., Textor M., Sutherland D.S.

    Last authors: D. S. Sutherland

    Organizations: Department of Applied Physics, Chalmers UniVersity of Technology, S-41296 Göteborg, Sweden, iNANO Center, UniVersity of Aarhus, Aarhus DK-8000, Denmark, and Laboratory of Surface Science and Technology, Department of Materials, ETH Zurich, Wolfgang Pauli Strasse 10, Zurich CH-8093, Switzerland

    Country: Switzerland

    Large area nanopatterns of functional proteins are demonstrated. A new approach to analyze atomic force microscopy height histograms is used to quantify protein and antibody binding to nanoscale patches. Arrays of nanopatches, each containing less than 40 laminin molecules, are shown to be highly functional binding close to 1 monoclonal anti-laminin IgG (site by IKVAV sequence) or 3-4 polyclonal anti-laminin IgG’s per surface bound laminin. Complementary quartz crystal microbalance measurements indicate higher functionality at nanopatches than on homogeneous surfaces.