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    Layer-by-layer assembly of type I collagen and chondroitin sulfate on aminolyzed PU for potential cartilage tissue engineering application

    Year: 2012

    Journal: Applied Surface Science Volume 258, Issue 24, 1 October 2012, Pages 9918–9925, 20130117

    Authors: Xianyun He a b c, Yingjun Wang a b c, Gang Wu a b c

    Last authors: Gang Wu

    Organizations: a School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China b National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China c Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou 510006, China

    Country: china

    In this paper, a two-step method was used to synthesize a biodegradable polyurethane (PU) composed of l-lysine ethyl ester diisocyanate (LDI), poly(ɛ-caprolactone) diols (PCL-diol) and 1,4:3,6-dianhydro-d-sorbitol (isosorbide). Amino groups were introduced onto the surface of the PU membrane by an amination reacting with 1,3-propanediamine to produce polycationic substratum. And then, type I collagen (Col) and chondroitin sulfate (CS) were deposited alternately on the polycationic substratum through layer-by-layer (LBL) assembly technology. The FTIR and 1H NMR results showed that the polyurethane was successfully synthesized. Rhodamine B isothiocyanate (RBITC) fluorescence spectrum indicated that amino groups were successfully introduced onto the PU surface. The results of quartz-crystal microbalance (QCM) and RBITC-Col fluorescence spectroscopy monitoring the LBL assemble process presented that the Col/CS deposited alternately on the PU surface. X-ray photoelectron spectroscopy (XPS) results displayed that the CS deposited on the PU surface as well. The surface of the assembled PU became even smoother observed from the surface morphology by atomic force microscopy (AFM) imaging. The hydrophilicity of the PU membrane was greatly enhanced though the modification of LBL assembly. The PU modified with the adsorption of Col/CS may be a potential application for cartilage tissue engineering due to its created mimicking chondrogenic environment.