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    Layer-by-layer coating of photoactive polymers for biomedical applications

    Year: 2011

    Journal: Surface and Coating technology 2012, 206 (8-9) pp 2446-2453, 20121211

    Authors: Valeria Chiono, Irene Carmagnola, Piergiorgio Gentile, Francesca Boccafoschi, Chiara Tonda-Turo, Mirko Ballarini, Ventsislava Georgieva, George Georgiev, Gianluca Ciardelli

    Organizations: Department of Mechanics, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy; Department of Clinical and Experimental Medicine, University of Eastern Piedmont Chemistry, Via Solaroli 17, 28100 Novara, Italy; Department of Physics, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy; Laboratory of Water-soluble polymers, polyelectrolytes and biopolymers, Faculty of Chemistry, University of Sofia James Bourchier Blvd 1, 1164 Sofia, Bulgaria

    Layer-by-layer (LbL) assembly is a versatile technique for the development of multilayered films with tailored characteristics at the nanometer scale. In this work, photoactive nanostructured films were prepared by LbL assembly of biocompatible photozymes: an anionic zwitterionic copolymer, poly(sodium styrene sulfonate-co-vinyl naphtalene-co-3-dimethyl(methacryloylethyl) ammonium propane sodium sulfonate; ZI) and a cationic polyelectrolyte, chitosan-g-fluorescein (CHFL) on a genipin-crosslinked gelatin substrate (G_GP). The correct organization of the multilayered coating was demonstrated by the static contact angle values, showing an alternate trend. FTIR–ATR spectra showed the main absorption bands of ZI, such as asymmetric (1125 and 1180 cm- 1) and symmetric (1036 cm- 1) OSO stretching and SOC stretching (1009 cm- 1), also confirmed by Energy-dispersive X-ray spectroscopy (EDS) spectra. UV–vis spectra showed the typical fluorescein absorbance peak of CHFL after the 8th layer, which intensity increased with increasing the even layer number. The absorption peak of G_GP (605–610 nm) probably masked the weak FL absorbance for coated samples with a lower layer number than 8. Scanning electron microscopy (SEM) results suggested the agglomerate presence on the coated surface. The obtained nanostructured films are expected to be promising candidates for carrying out efficient electron transfer process, responsible for the anti-microbial activity (ZI) or the osteointegration ability (CHFL) of the coating.