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    Light-regulated release of liposomes from phospholipid membranes via photoresponsive polymer–DNA conjugates

    Year: 2006

    Journal: Soft Matter, 2006, 2, 710-715, 20120104

    Authors: Benkoski J:J 1 *, Jesorka A 2, Edvardsson M. 1, Höök F. 3

    Last authors: Fredrik Höök

    Organizations: 1 Chalmers University of Technology, Applied Physics Department, 41296 Gothenburg, Sweden. E-mail: jason.benkoski@nist.gov; Fax: 301-975-3928 2 Chalmers University of Technology, Department of Physical Chemistry, 41296 Gothenburg, Sweden 3 Lund University, Department of Solid State Physics, 22100 Lund, Sweden

    Country: Sweden, Sverige

    A method for releasing tethered liposomes from a supported lipid bilayer in response to a light stimulus is described. The tethering is accomplished through the hybridization of end-functionalized DNA that resides on both the supported lipid bilayer and liposome surfaces. Normally consisting of cholesterol or lipid tails, the end group is replaced in this study by a photoresponsive polymer that partitions into lipid bilayers at physiological pH. When exposed to UV light, it undergoes excited state proton transfer with water. The ensuing increase in polarity increases the solubility of the polymer in the aqueous phase. Quartz crystal microbalance with dissipation monitoring (QCM-D) and fluorescence microscopy have been used to record both the construction of the vesicle assembly and the subsequent response to UV light. It is found that the critical flow rate for vesicle release is reduced when buffer flow is performed in conjunction with UV exposure.