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    Lysozyme interaction with poly(HEMA)-based hydrogel

    Year: 2006

    Journal: Biomaterials 27 (2006) 1341–1345, 20100827

    Authors: Lord M.S., Stenzel M.H., Simmons A., Milthorpe B.K.

    Last authors: Bruce K. Milthorpe

    Organizations: Graduate School of Biomedical Engineering, The University of New South Wales, Sydney, NSW 2052 Australia, Centre for Advanced Macromolecular Design, School of Chemical Engineering and Industrial Chemistry, The University of New South Wales, Sydney, NSW 2052, Australia

    Country: Australia

    Lysozyme interaction with an acrylic-based hydrogel,poly(2-hydroxyethyl methacrylate) co-methacrylic acid (P(HEMA-MAA)),was investigated using a combination of quartz crystal microbalance with dissipation (QCM-D),surface plasmon resonance (SPR) and dual polarisation interferometry (DPI). This combination of techniques demonstrated that lysozyme initially absorbed into the hydrogel matrix and displaced water from the hydrogel while subsequent lysozyme additions were adsorbed onto the surface of the hydrogel material. QCM-D,being sensitive to bound water, showed an overall decrease in mass and stiffening of the layer after lysozyme addition. SPR,a water insensitive technique,showed a net mass increase after addition of lysozyme and buffer rinses. DPI showed that the first exposure of lysozyme to P(HEMA-MAA) was consistent with lysozyme absorption while subsequent lysozyme exposures were consistent with lysozyme adsorption.