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    Microcontact imprinted surface plasmon resonance sensor for myoglobin detection

    Year: 2013

    Journal: Materials Science and Engineering, C, Volume 33, Issue 7, October 2013, Pages 3609–3614, 20131119

    Authors: Bilgen Osman, Lokman Uzun, Necati Beşirli, Adil Denizli

    Organizations: Uludag University, Department of Chemistry, Bursa, Turkey, Hacettepe University, Department of Chemistry, Ankara, Turkey

    In this study,we prepared surface plasmon resonance (SPR) sensor using the molecular imprinting technique formyoglobin detection in human serum. For this purpose,wesynthesizedmyoglobin imprinted poly(hydroxyethylmethacrylate-N-methacryloyl-L-tryptophanmethyl ester) [poly(HEMA-MATrp)] nanofilm on the surface of SPRsensor.We also synthesized non-imprinted poly(HEMA-MATrp) nanofilmwithoutmyoglobin for the control experiments.The SPR sensor was characterized with contact angle measurements, atomic force microscopy, X-rayphotoelectron spectroscopy, and ellipsometry.Weinvestigated the effectiveness of the sensor using the SPR system.We evaluated the ability of SPR sensor to sense myoglobin with myoglobin solutions (pH 7.4, phosphatebuffer) in different concentration range and in the serum taken from a patient with acutemyocardial infarction.We found that the Langmuir adsorption model was the most suitable for the sensor system. The detection limitwas 87.6 ng/mL. In order to show the selectivity of the SPR sensor, we investigated the competitive detection ofmyoglobin, lysozyme, cytochrome c and bovine serum albumin. The results showed that the SPR sensor has highselectivity and sensitivity for myoglobin.