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    Microcontact printing of polyelectrolytes on PEG using an unmodified PDMS stamp for micropatterning nanoparticles, DNA, proteins and cells

    Year: 2012

    Journal: Soft Matter 2012, 8 (29) pp 7630-7637, 20121211

    Authors: Zhibin Wang , Peipei Zhang , Brett Kirkland , Yingru Liu and Jingjiao Guan

    Organizations: Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Integrative NanoScience Institute, Florida State University, 2525 Pottsdamer Street, Tallahassee, Florida, 32310, USA.

    A facile microcontact printing method has been developed based on directly printing polyelectrolytes on a glass or polystyrene surface coated with poly(ethylene glycol) (PEG) silane using an unmodified poly(dimethyl siloxane) (PDMS) stamp. The method is applicable to a variety of polyelectrolytes including poly(allylamine hydrochloride) (PAH), poly(diallyldimethylammonium chloride) (PDAC), branched and linear poly(ethylene imine) (PEI), poly-L-lysine (PLL), chitosan, double stranded DNA, and poly(sodium 4-styrene sulfonate) (PSS). The printed polyelectrolyte structures, which include monolayer, bilayer, and stretched molecular bundles, are stable in aqueous solutions and have been used as templates for micropatterning quantum dot nanoparticles, DNA, proteins, and live cells.