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    Miscibility in Binary Monolayers of Phospholipids and Linker Lipid

    Year: 1999

    Journal: Langmuir 1999, 15, 2516-2524, 20111221

    Authors: E. Györvary, W. M. Albers, and J. Peltonen

    Organizations: Center for Ultrastructure Research, Universität für Bodenkultur Wien, Gregor-Mendel-Strasse 33, A-1180 Vienna, Austria, Technical Research Centre of Finland, Chemical Technology, P.O. Box 14021, FIN-33101 Tampere, Finland, and Department of Physical Chemistry, Åbo Akademi University, Porthaninkatu 3-5, FIN-20500 Turku, Finland

    We studied the miscibility in binary lipid matrixes made by the Langmuir-Blodgett (LB) technique. The components in the lipid matrix were N-(β-maleimidocaproyl)-dipalmitoyl phosphatidylethanolamine (DPPE-EMC; biofunctionalized linker lipid) and a phospholipid. Three different matrix phospholipids were used: 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine (DPPE), 1,2-dimyristoyl-sn-glycero-3-phosphatidylethanolamine (DMPE), and 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC). The phase-transition temperature of the linker lipid as determined by Fourier transform infrared spectroscopy was 45 °C. The surface potential of the linker lipid, 290 mV at pH 6.8, was clearly smaller than the values observed for pure phospholipids. Clear evidence of the miscibility could not be obtained from the surface pressure-area isotherms.Onthe contrary, Brewster angle microscopy (BAM) enabled a visual investigation of the miscibility and domain morphology. The best miscibility was obtained for DPPC/DPPE-EMC matrixes but only to some extent for DPPE/DPPE-EMC and DMPE/DPPE-EMC matrixes. Atomic force microscopy on solid supported LB films showed domains similar to the BAM images of Langmuir monolayers.