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    Mixed Monolayers of Amphotericin B-Dipalmitoyl Phosphatidyl Choline: Study of Complex Formation

    Year: 2002

    Journal: Langmuir 2002, 18, 2817-2827, 20111221

    Authors: J. Miones, Jr., J. Miones, O. Conde, J. M. Rodriguez Patino, and P. Dynarowiczatka

    Organizations: University of Santiago de Compostela, Faculty of Pharmacy, Department of Physical Chemistry, Campus Sur, 15706 Santiago de Compostela, Spain, University of Seville, Faculty of Chemistry, Department of Chemical Engineering, c/Prof. Garcia Gonzalez, s/n. 41012 Seville, Spain, and Jagiellonian University, Faculty of Chemistry, Ingardena 3, 30-060 Krakow, Poland

    Mixed Langmuir monolayers of amphotericin B (AmB) and dipalmitoyl phosphatidyl choline (DPPC) were investigated by recording surface pressure-area (π-A) isotherms in addition to Brewster angle microscopy. The analyses of π-A and compressional modulus curves indicate the existence of interactions in the AmB-DPPC system; the greatest were found to occur for a ca. 2:1 AmB/DPPC mixture. For this mixed monolayer (XAmB = 0.66), the formation of a stable complex, composed of two horizontally oriented AmB molecules and one DPPC molecule in a vertical position, is suggested. For mixtures containing AmB in excess as compared to the stoichiometric mixture for the complex formation (XAmB > 0.66), the filmforming components are miscible and the mixed monolayer consists of AmB-DPPC complexes together with horizontally (at surface pressures below the first transition) or vertically (at pressures above this transition) oriented free AmB molecules. On the other hand, mixtures of AmB-DPPC complex and DPPC in excess (at XAmB < 0.66) were found to be miscible at surface pressures below the second transition. However, the system becomes immiscible at higher surface pressures.