Year: 2006
Journal: Colloids and Surfaces B: Biointerfaces, Volume 53, Issue 1, 1 November 2006, Pages 37-50, 20111221
Authors: Kazuki Hoda, Hideya Kawasaki, Norio Yoshino, Chien-Hsiang Chang, Yoko Morikawa, Gohsuke Sugihara and Osamu Shibat
Organizations: Division of Biointerfacial Science, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, Department of Chemistry, Graduate School of Sciences, Kyushu University, 6-1-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan, Department of Industrial Chemistry, Faculty of Engineering, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan, Institute of Colloid and Interface Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan, Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan 70101, Republic of China, Department of Chemistry, Faculty of Science, Fukuoka University, 8-19-1 Nanakuma, Jyonan-ku, Fukuoka 814-0180, Japan
Two-component Langmuir monolayers formed on 0.02 M Tris buffer solution (pH 7.4) with 0.13 M NaCl at 298.2 K were investigated for two different fluorinated-hydrogenated hybrid amphiphiles (F6PH5PPhNa and F8PH5PPhNa or F6 and F8, respectively) with DPPC. Surface pressure (π), surface potential (ΔV) and dipole moment (μ
) as a function of molecular surface area (A) were measured by employing the Whilhelmy method and an ionizing electrode method. From the A– and ΔV–XF6 (or XF8) curves, partial molecular surface area (PMA) and apparent partial molecular surface potential (APSP) were determined as a function of surface mole fraction (XFn) at discrete surface pressures. Then, the behavior of occupied surface areas and surface potentials of the respective components could be made clearer. Compressibility (Cs), elasticity 
, and excess Gibbs energy (ΔG(ex)) as a function of XF6 (or XF8) were estimated at definite pressures. These physico-chemical parameters were found to reflect the mechanical strength of monolayer films formed. The regular solution theory being applied to ΔG(ex), the activity coefficients (f) as well as the interaction parameter (Ip) between DPPC and two hybrid amphiphiles in the binary monolayers were evaluated. Ip values thus obtained indicated that F8 molecules interact more strongly with DPPC molecules than F6. Moreover, in order to better understand the morphological monolayer state, Langmuir–Blodgett (LB) films made from DPPC and fluorinated-hydrogenated hybrid amphiphiles were examined by atomic force microscopy (AFM). The miscibility of the two components in the monolayer state is evidenced by these thermodynamic quantities and AFM observations. Furthermore, AFM images demonstrated that F8 could more effectively disperse the ordered domains of DPPC than F6.
