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    Modification of Electrodes with Self-Assembled Hydrophobin Layers

    Year: 2001

    Journal: J. Phys. Chem. B 2001, 105, 9772-9777, 20111221

    Authors: Renata Bilewicz, Jaroslaw Witomski, Angeline Van der Heyden, Denis Tagu, Beatrice Palin, and Ewa Rogalska

    Organizations: Department of Chemistry, University of Warsaw, ul. Pasteura 1, 02093 Warsaw, Poland, Equipe de Physico-chimie des Colloides, UMR 7565 CNRS/Universite Henri Poincare, Nancy I, Faculte des Sciences, BP 239, 54506 Vandoeuvre-les-Nancy, Cedex, France, UMR IaM 1136 INRA/Universite Henri Poincare, INRA-Nancy, 54280 Champenoux, France

    The small fungal protein hydrophobin from Pisolithus tinctorius (HYDPt-1) has been used to modify electrode surfaces by self-assembly from aqueous solutions. Voltammetric experiments performed with Fe(CN)63- as an electrochemical probe have shown that hydrophobin can be immobilized on hydrophobic surfaces of a glassy carbon electrode (GCE), or on a thin mercury film electrode (TMFE), or on hydrophilic surfaces such as a gold electrode (GE). Higher efficiency of coverage is obtained for GCE or TMFE. The hydrophobinmodified electrodes were in turn functionalized with the electroactive ubiquinone (Q10), quinone (Q0), and azobenzene. While Q10 was adsorbed on the surface of the hydrophobin layer, smaller molecules, azobenzene and Q0, penetrated into the pores of the layer. In all cases, a stable attachment of the electroactive reagents to the electrode via the hydrophobin was achieved.