Contact
    Start Publications Multilayer film assembled from charged derivatives of chitosan: ...
    ←  Publication Finder
    QSense

    Multilayer film assembled from charged derivatives of chitosan: Physical characteristics and biological responses

    Year: 2012

    Journal: Journal of Colloid and Interface Science, Volume 376, Issue 1, 15 June 2012, Pages 177–188, 20120618

    Authors: Wilaiporn Graisuwan a, b, Oraphan Wiarachai a, b, Cheeraporn Ananthanawat c,  Songchan Puthong d, Suphan Soogarun e, Suda Kiatkamjornwong f, Voravee P. Hoven g

    Last authors: Voravee P. Hoven

    Organizations: •a Program in Petrochemistry and Polymer Science, Faculty of Science, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330, Thailand •b Center for Petroleum, Petrochemicals, and Advanced Materials, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330, Thailand •c Program in Macromolecular Science, Faculty of Science, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330, Thailand •d The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330, Thailand •e Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330, Thailand •f Department of Imaging and Printing Technology, Faculty of Science, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330, Thailand •g Organic Synthesis Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330, Thailand

    Country: Thailand

    Polyelectrolyte multilayer films were successfully assembled from each of the three charged derivatives of chitosan; N-[(2-hydroxyl-3-trimethylammonium)propyl]chitosan chloride (HTACC), N-succinyl chitosan (SCC) and N-sulfofurfuryl chitosan (SFC), paired with one of the two oppositely charged polyelectrolytes, poly(acrylic acid) (PAA) and poly(allylamine hydrochloride) (PAH) on surface-treated poly(ethylene terephthalate) (treated PET) substrates by alternate layer-by-layer adsorption. Surface coverage and wettability of the multilayer films were determined by AFM and water contact angle measurements, respectively. Analysis by quartz crystal balance with dissipation (QCM-D) has suggested that all multilayer films are relatively rigid and have a high water content associated within their structures, accounting for up to 85–90% (w/w) for films having 7–10 layers. In vitro cytocompatibility tests for the fibroblast-like L929 cell line revealed a slight dependency for cell adhesion and proliferation on the outermost layer. The multilayer film containing HTACC exhibited moderate antibacterial activity against E. coli and S. aureus. Bearing negative charges, the multilayer films terminating with SFC and having at least 10 layers were capable of suppressing the adsorption of plasma proteins and platelet adhesion at a comparable level to the multilayer film assembled from heparin, a well-known antithrombogenic polymer.