Contact
    Start Publications Phosphorylcholine-Modified Chitosan Films as Effective ...
    ←  Publication Finder
    QSense

    Phosphorylcholine-Modified Chitosan Films as Effective Promoters of Cell Aggregation: Correlation Between the Films Properties and Cellular Response

    Year: 2015

    Journal: MACROMOLECULAR BIOSCIENCE, Vol. 15, p 490-500, 20150526

    Authors: Qi, Baowen; Kujawa, Piotr; Toita, Sayaka; Beaune, Gregory; Winnik, Francoise M.

    Organizations: Univ Montreal, Fac Pharm, Montreal, PQ H3C 3J7, Canada; Univ Montreal, Dept Chim, Montreal, PQ H3C 3J7, Canada; Natl Inst Mat Sci, World Premier Int WPI Res Ctr Initiat, Int Ctr Mat Nanoarchitecton MANA, Tsukuba, Ibaraki 3050044, Japan

    This study describes chitosan-phosphorylcholine (CH-PC) films able to support the formation of cell aggregates (spheroids), which are important for tissue engineering and pharmacological studies. The surface topography, charge, thickness, and rheology of CH-PC thin films were characterized by AFM, zeta-potential measurements, SPR spectroscopy, and QCM-D measurements. The CH-PC films are highly hydrated gels, independently of the level of PC incorporation (15-40 mol-% PC/glucosamine units). QCM-D studies established that the amount of fibrinogen adsorbed on CH-PC films decreased with increasing PC content. CH-PC surfaces underwent a transition from moderately cell-adhesive (CH-PC15) to non-adhesive (CH-PC40). Optical micrographs of HUVEC and MCF-7 cell lines cultured on CH-PC surfaces showed that they form spheroids on CH-PC25 and CH-PC40 films.