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    Probing the Functionalization of Gold Surfaces and Protein Adsorption by PM-IRRAS

    Year: 2011

    Journal: ChemPhysChem, 2011, 12 (9), 1736-1740, 20131009

    Authors: Dr. Rodrigo Marques de Oliveira, Prof. Dr. Jacqueline Ferreira, Prof. Dr. Marcos J. L. Santos, Prof. Dr. Roberto M. Faria, Prof. Dr. Osvaldo N. Oliveira Jr.

    Organizations: Instituto de Física de São Carlos, Universidade de São Paulo, P.O. Box 369, 13566-590, São Carlos, São Paulo, Brazil; Instituto de Química e Geociências, Universidade Federal de Pelotas, P.O. Box 354, 96010-900, Pelotas, Rio Grande do Sul, Brazil; Instituto de Química, Universidade Federal do Rio Grande do Sul, 91501-970, Porto Alegre, Rio Grande do Sul, Brazil

    The control of morphology and coating of metal surfaces is essential for a number of organic electronic devices including photovoltaic cells and sensors. In this study, we monitor the functionalization of gold surfaces with 11-mercaptoundecanoic acid (MUA, HS(CH2)10CO2H) and cysteamine, aiming at passivating the surfaces for application in surface plasmon resonance (SPR) biosensors. Using polarization-modulated infrared reflection–absorption spectroscopy (PM-IRRAS), cyclic voltammetry, atomic force microscopy and quartz crystal microbalance, we observed a time-dependent organization process of the adsorbed MUA monolayer with alkyl chains perpendicular to the gold surface. Such optimized condition for surface passivation was obtained with a systematic search for experimental parameters leading to the lowest electrochemical signal of the functionalized gold electrode. The ability to build supramolecular architectures was also confirmed by detecting with PM-IRRAS the adsorption of streptavidin on the MUA-functionalized gold. As the approaches used for surface functionalization and its verification with PM-IRRAS are generic, one may now envisage monitoring the fabrication of tailored electrodes for a variety of applications.