Contact
    Start Publications Promotion of osteoblast proliferation on complex ...
    ←  Publication Finder
    QSense

    Promotion of osteoblast proliferation on complex coacervation-based hyaluronic acid – recombinant mussel adhesive protein coatings on titanium

    Year: 2010

    Journal: Biomaterials, Volume 31, Issue 6, February 2010, Pages 1080-1084, 20100827

    Authors: Hwang D.S 1, Waite J.H. 1 2, Tirrell M. 1 3

    Last authors: Matthew Tirrella

    Organizations: 1 Material Research Laboratory, University of California, Santa Barbara, California 93106, USA 2 Department of Molecular, Cell, and Developmental Biology, University of California, Santa Barbara, California 93106, USA 3 Department of Chemical Engineering, University of California, Santa Barbara, California 93106, USA

    Country: USA, US, United States of America

    Many biological polyelectrolytes are capable of undergoing a fluid–fluid phase separation known as complex coacervation. Coacervates were prepared using hyaluronic acid (HA) and a recombinant fusion protein consisting of mussel adhesive motifs and the RGD peptide (fp-151-RGD). The low interfacial energy of the coacervate was exploited to coat titanium (Ti), a metal widely used in implant materials. The coacervate effectively distributed both HA and fp-151-RGD over the Ti surfaces and enhanced osteoblast proliferation. Approximately half of total fp-151-RGD and HA in the solution transferred to the titanium surface within 2 h. Titanium coated with coacervates having high residual negative surface charge showed the highest cell proliferation of preosteoblast cells (MC-3T3) compared to the treatments tested. Indeed, MC-3T3 cells on complex coacervate coated titanium foils exhibited over 5 times greater cell proliferation than bare, HA coated or fp-151-RGD coated titanium.