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    Quartz-crystal microbalance-dissipation technique for the study of initial adsorption of fibronectin onto tresyl chloride-activated titanium

    Year: 2005

    Journal: Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2005, Volume 73B, Issue 2 , Pages 271 - 276, 20100827

    Authors: Hayakawa T. 1 *, Yoshinari M. 2, Nemoto K. 1

    Last authors: K. Nemoto

    Organizations: 1Department of Dental Materials, Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, 2-870-1, Sakaecho-nishi, Matsudo, Chiba 271-8587, Japan 2Department of Dental Materials Science and Oral Health Science Center, Tokyo Dental College, 1-2-2 Masago, Mihamaku, Chiba 261-8502, Japan

    Country: Japan

    The immobilization of cell-adhesive proteins onto titanium implants improves biological response at the implant-tissue interface. Previous studies demonstrated the easy and direct attachment of fibronectin onto titanium with the use of a 2,2,2-trifluoroethanesulfonyl chloride (tresyl chloride) activation technique. The present study investigated the initial adsorption behavior of fibronectin on tresyl chloride-activated titanium by the quartz-crystal microbalance-dissipation (QCM-D) technique. The crystal resonant frequency and the dissipation shift of the oscillator were simultaneously measured by the injection of fibronectin/phosphate-buffered saline solution (pH = 7.4). The tresyl chloride-activated titanium surface showed a faster and greater decrease in frequency than that of untreated titanium, indicating that a greater amount of fibronectin was adsorbed in the former case during a 120-min adsorption. The dissipation-frequency plots revealed that, during the initial stage of adsorption, the bond between fibronectin and tresyl chloride-activated titanium is stronger than that between fibronectin and untreated titanium. The QCM-D technique can provide new insights into the adsorption mechanism of fibronectin.