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    Quartz crystal microbalance with dissipation monitoring of supported lipid bilayers on various substrates

    Year: 2010

    Journal: Nature Protocols 5, - 1096 - 1106 (2010), 20100827

    Authors: Cho N-J 1 2, Frank C.W. 2, Kasemo B. 3, Höök F. 3

    Last authors: Fredrik Höök

    Organizations: 1. Division of Gastroenterology and Hepatology, Department of Medicine, Stanford University, Stanford, California, USA. 2. Department of Chemical Engineering, Stanford University, Stanford, California, USA. 3. Department of Applied Physics, Chalmers University of Technology, Gothenburg, Sweden.

    Country: Sweden

    Supported lipid bilayers (SLBs) mimic biological membranes and are a versatile platform for a wide range of biophysical research fields including lipid–protein interactions, protein–protein interactions and membrane-based biosensors. The quartz crystal microbalance with dissipation monitoring (QCM-D) has had a pivotal role in understanding SLB formation on various substrates. As shown by its real-time kinetic monitoring of SLB formation, QCM-D can probe the dynamics of biomacromolecular interactions. We present a protocol for constructing zwitterionic SLBs supported on silicon oxide and titanium oxide, and discuss technical issues that need to be considered when working with charged lipid compositions. Furthermore, we explain a recently developed strategy that uses an amphipathic, α-helical (AH) peptide to form SLBs on gold and titanium oxide substrates. The protocols can be completed in less than 3 h.