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    Rapid and Complete Enzyme Hydrolysis of Lignocellulosic Nanofibrils

    Year: 2012

    Journal: ACS Macro Lett. 2012, 1, 1321−1325, 20130116

    Authors: Raquel Martin-Sampedro †‡, Ilari Filpponen †, Ingrid C. Hoeger §, J. Y. Zhu ∥, Janne Laine †, Orlando J. Rojas * † §

    Last authors: Orlando J. Rojas

    Organizations: †School of Chemical Technology, Department of Forest Products Technology, Aalto University, 00076 Aalto, Finland §Department of Forest Biomaterials, North Carolina State University, Raleigh, North Carolina 27695, United States ∥Forest Products Laboratory, USDA Forest Service, Madison, Wisconsin 53726, United States

    Country: Finland, USA, US, United States, United States of America, America

    Rapid enzymatic saccharification of lignocellulosic nanofibrils (LCNF) was investigated by monitoring nanoscale changes in mass via quartz crystal microgravimetry and also by measuring reducing sugar yields. In only a few minutes LCNF thin films were completely hydrolyzed upon incubation in multi- component enzyme systems. Conversion to sugars and oligosac- charides of LCNF dispersed in water occurred in about 4 h (50 °C, pH 5). In contrast, a conversion of only 57% was observed for partially crystalline cellulose (Avicel) after 9 h, under same experimental conditions. Under conditions of high enzyme loading the presence of residual lignin and other macromolecules in the cell wall of LCNF did not appear to affect negatively the recorded high hydrolysis rates. Overall, our findings suggest that deconstruction of the cell wall to nanofibrils is an effective pretreatment to facilitate rapid and complete cellulose bioconversion.