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    Rapid three-dimensional biointerfaces for real-time immunoassay using hIL-18BPa as a model antigen

    Year: 2005

    Journal: Biomaterials 26 (2005) 7514-7523, 20100827

    Authors: Carrigan wn D., Scott G., Tabrizian M.

    Last authors: Maryam Tabrizian

    Organizations: Biomedical Engineering Department, McGill University, 3775 University St., Duff Medical Building, rm 316, Montreal, Que., Canada H3A 2B4, MDS Pharma Services, 2350 Cohen St., St. Laurent, Que., Canada H4R 2N6, Faculty of Dentistry, Centre for Biorecognition and Biosensors, McGill Institute for Advanced Materials, Montreal, Que., Canada H3A 2T8

    Country: Canada

    With the goal of designing a rapid and affordable system of real-time immune monitoring for future diagnostic applications in sepsis, we have developed a biointerface composed of polyethyleneimine (PEI) and carboxymethylcellulose (CMC) to provide a means of prompt and facile immunoassay. Biointerface assembly is complete within 30 min, with all preparation performed and monitored within the measurement chamber of a quartz crystal microgravimetry with dissipation (QCM-D) sensor. Optimised biointerface composition, as determined bythe mass of antibodyimmobilised, the level of antigen detection, and the amount of nonspecific binding of human serum albumin, was determined to consist of a 4.0 mg/mL CMC hydrogel layer cross-linked to a 0.5 mg/ mL PEI sub-layer. Tapping mode atomic force microscopy (AFM) in liquid demonstrates highly uniform and smooth surfaces using these hydrogels. Sensitivity of the biointerface for rhIL-18BPa is 400 ng/mL, with detection of 1 mg/mL achievable following 25 surface regenerations. Performance of the biointerface is verified using surface plasmon resonance (SPR), demonstrating the ability of the biointerface to be applied across platforms.