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    Real-Time Analysis of Porphyrin J-Aggregation on a Plant-Esterase-Functionalized Surface Using Quartz Crystal Microbalance with Dissipation Monitoring

    Year: 2014

    Journal: Langmuir, 2014, 30 (33), pp 9962–9971, 20141008

    Authors: Limin Yang †, Lei Jiang †, Weijing Yao ‡§, Junling Liu †, and Juan Han †

    Last authors: Juan Han

    Organizations: † State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, Shandong 266555, P. R. China ‡ Evidence Identification Center, Public Security Bureau, Chongqing 400021, P. R. China § Engineering Research Center for Criminal Investigation, Chongqing 400021, P. R. China

    Country: China

    The J-aggregation of meso-tetra (4-sulfonatophenyl) porphine (TPPS4) on a plant-esterase-functionalized surface in a 1:1 v/v mixture of 0.05 M HCl/ethanol (pH ∼1.38) was analyzed in real time using a quartz crystal microbalance with dissipation monitoring (QCM-D). Simultaneous changes in frequency (Δf) and energy dissipation (ΔD) correlated well with mass and structural changes during the sequential phases of slow nucleation, rapid aggregation, and equilibration in J-aggregation. The time-dependent mass adsorption could be quantitatively analyzed with a model, which integrated two simple equations obtained when the surface concentration of TPPS4 (ΓTPPS4) was below and above the critical aggregation surface concentration (CASC). This study provides a new view for the protein-induced J-aggregation process, which may be helpful for understanding the interactions of self-assembled nanostructures with biomolecules.