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    Real-time QCM-D monitoring of cellular responses to different cytomorphic agents

    Year: 2011

    Journal: Biosensors and Bioelectronics, Volume 26, Issue 7, 15 March 2011, Pages 3207-3212, 20110317

    Authors: Fatisson J. 1, Azari F 2,  Tufenkji N 1

    Last authors: Nathalie Tufenkji

    Organizations: 1 Department of Chemical Engineering, McGill University, Montreal, Quebec H3A 2B2, Canada 2 Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, VT 05403, USA

    Country: Canada

    Quartz crystal microbalance with dissipation monitoring ( QCM -D) is used for real-time in situ detection of cytoskeletal changes in live primary endothelial cells in response to different cytomorphic agents; namely, the surfactant Triton-X 100 (TX-100) and bacterial lipopolysaccharide (LPS). Reproducible dissipation versus frequency (Df) plots provide unique signatures of the interactions between endothelial cells and cytomorphic agents. While the QCM -D response for TX-100 can be described in two steps (changes in the osmotic pressure of the medium prior to observing the expected cell lysis), LPS results in a different single-phase signal. A complementary analysis is carried out to evaluate the possible competitive effects of TX-100 and LPS through the QCM -D response to BAEC stress by analyzing the Df plots obtained. Experiments with non-toxic components (fibronectin or serum) produce a different QCM -D response than that observed for the toxic chemicals, suggesting the use of Df plot signatures for the possible differentiation between cytotoxic or non-cytotoxic effects. Observations obtained by QCM -D signals are confirmed by conducting fluorescence microscopy at the same time. Our results show that a fast (few minutes) sensing response can be obtained in situ and in real-time. The conclusions from this study suggest that QCM -D can potentially be used in biodetection for applications in drug screening tests and diagnosis.