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    SAM-based cell transfer to photopatterned hydrogels for microengineering vascular-like structures

    Year: 2011

    Journal: Biomaterials, Volume 32, Issue 30, October 2011, Pages 7479-7490, 20111207

    Authors: Sadr N. 1 2 3 4, Zhue M., Osaki T 6, Kakegawa T 6, Yang Y. 7, Moretti M 8, Fukuda J 1 6, Khademhosseini A. 1 2 3

    Last authors: Ali Khademhosseini

    Organizations: 1 Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA 2 Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA 3 Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02139, USA 4 Bioengineering Department, Politecnico di Milano, 20131 Milan, Italy 5 Department of Biology, Mount Holyoke College, South Hadley, MA 01075, USA 6 Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan 7 Department of Restorative Dentistry and Biomaterials, University of Texas Health Science Center, Houston, TX 77030, USA 8 Cell and Tissue Engineering Laboratory, IRCCS Istituto Ortopedico Galeazzi, 20161 Milan, Italy

    Country: Italy, Japan, USA, US, United States, United States of America, America

    A major challenge in tissue engineering is to reproduce the native 3D microvascular architecture fundamental for in vivo functions. Current approaches still lack a network of perfusable vessels with native 3D structural organization. Here we present a new method combining self-assembled monolayer (SAM)-based cell transfer and gelatin methacrylate hydrogel photopatterning techniques for microengineering vascular structures. Human umbilical vein cell (HUVEC) transfer from oligopeptide SAM-coated surfaces to the hydrogel revealed two SAM desorption mechanisms: photoinduced and electrochemically triggered. The former, occurs concomitantly to hydrogel photocrosslinking, and resulted in efficient (>97%) monolayer transfer. The latter, prompted by additional potential application, preserved cell morphology and maintained high transfer efficiency of VE-cadherin positive monolayers over longer culture periods. This approach was also applied to transfer HUVECs to 3D geometrically defined vascular-like structures in hydrogels, which were then maintained in perfusion culture for 15 days. As a step toward more complex constructs, a cell-laden hydrogel layer was photopatterned around the endothelialized channel to mimic the vascular smooth muscle structure of distal arterioles. This study shows that the coupling of the SAM-based cell transfer and hydrogel photocrosslinking could potentially open up new avenues in engineering more complex, vascularized tissue constructs for regenerative medicine and tissue engineering applications.